Prevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Türkiye

Cryptosporidiosis, one of the main protozoan infections last century, is especially dangerous for calves and causes significant economic losses. This research was carried out to determine prevalence Cryptosporidium spp. by microscopic molecular methods subtypes in 100 up 6 months old with diarrhoea Siirt Province, Türkiye. As a result examination (Kinyoun’s acid-fast), oocysts were found 8 (8%) samples. nested PCR, 826-864 bp specific bands obtained 13 (13%) When DNA sequences SSU rRNA gene compared NCBI Basic Local Alignment Search Tool database, it determined that eight samples sequence analyses showed 100% similarity C. parvum , ryanae bovis The detection which has zoonotic importance this study, suggests may be source contamination other animals humans. Therefore, animal owners people close contact should informed about public health cryptosporidiosis.